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Neutrophil (mouse) Isolation Kit

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    Neutrophil (mouse) Isolation Kit
  • Neutrophil (mouse) Isolation Kit
Cat No: 601070
Assays - Miscellaneous
Cayman
€257.00
Price is excluding VAT and does not include packaging neither shipping

Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive an...

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: 1 ea

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Territorial Availability: Available through Bertin Pharma only in Europe
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Product Overview:
Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme, myeloperoxidase, neutrophil elastase, cathepsins, and the alpha- and beta-defensins. Neutrophils also form extracellular traps, web-like structures composed of DNA, histones, and various antimicrobial proteins that are extruded from the neutrophil in order to ensnare and kill extracellular pathogens. In mice, neutrophils comprise ~10% of circulating white blood cells, so unlike humans, it is usually not possible to isolate even one million neutrophils from a single mouse from peripheral blood. Cayman’s Neutrophil (mouse) Isolation Kit provides a streamlined method for isolating mouse neutrophils by peritoneal lavage. Included in this kit are all the reagents necessary for inducing a sterile inflammatory response in the peritoneal cavities of up to five mice, the density separation medium required to separate the neutrophils from other peritoneal leukocytes, and the fluorochrome-labeled antibodies required to assess the success of the isolation procedure by flow cytometry.
Neutrophils are short-lived inflammatory cells that are highly adept at ingesting and destroying bacteria as part of the first line of defense in the innate immune response to many pathogenic organisms. They produce an extensive arsenal of defensive antimicrobial proteins and peptides including lysozyme, myeloperoxidase, neutrophil elastase, cathepsins, and the alpha- and beta-defensins. Neutrophils also form extracellular traps, web-like structures composed of DNA, histones, and various antimicrobial proteins that are extruded from the neutrophil in order to ensnare and kill extracellular pathogens. In mice, neutrophils comprise ~10% of circulating white blood cells, so unlike humans, it is usually not possible to isolate even one million neutrophils from a single mouse from peripheral blood. Cayman’s Neutrophil (mouse) Isolation Kit provides a streamlined method for isolating mouse neutrophils by peritoneal lavage. Included in this kit are all the reagents necessary for inducing a sterile inflammatory response in the peritoneal cavities of up to five mice, the density separation medium required to separate the neutrophils from other peritoneal leukocytes, and the fluorochrome-labeled antibodies required to assess the success of the isolation procedure by flow cytometry.
Size: 1 ea
Shipping: wet ice
Stability: Store at -20 degrees; shelf life 365 days maximum after production
Custom Code: 3822000002
UNSPSC code: 41116104

Brummer, E., McEwen, J.G., and Stevens, D.A. Fungicidal activity of murine inflammatory polymorphonuclear neutrophils: Comparison with murine peripheral blood PMN. Clin Exp Immunol 66(3) 681-690 (1986).

Baron, E.J., and Proctor, R.A. Elicitation of peritoneal polymorphonuclear neutrophils from mice. J Immunol Methods 49(3) 305-313 (1982).

Hasenberg, M., Köhler, A., Bonifatius, S., et al. Rapid immunomagnetic negative enrichment of neutrophil granulocytes from murine bone marrow for functional studies in vitro and in vivo. PLoS One 6(2) e17314 (2011).

Luo, Y., and Dorf, M.E. Isolation of mouse neutrophils. Curr Protoc Immunol 3(Unit 3.20) 1-6 (2001).

Boxio, R., Bossenmeyer-Pourié, C., Steinckwich, N., et al. Mouse bone marrow contains large numbers of functionally competent neutrophils. J Leukoc Biol 75(4) 604-611 (2004).

Daley, J.M., Thomay, A.A., Connolly, M.D., et al. Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J Leukoc Biol 83(1) 64-70 (2008).

Cayman Chemical's mission is to help make research possible by supplying scientists worldwide with the basic research tools necessary for advancing human and animal health. Our utmost commitment to healthcare researchers is to offer the highest quality products with an affordable pricing policy.

Our scientists are experts in the synthesis, purification, and characterization of biochemicals ranging from small drug-like heterocycles to complex biolipids, fatty acids, and many others. We are also highly skilled in all aspects of assay and antibody development, protein expression, crystallization, and structure determination.

Over the past thirty years, Cayman developed a deep knowledge base in lipid biochemistry, including research involving the arachidonic acid cascade, inositol phosphates, and cannabinoids. This knowledge enabled the production of reagents of exceptional quality for cancer, oxidative injury, epigenetics, neuroscience, inflammation, metabolism, and many additional lines of research.

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ISO Guide 34:2009

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